CRISPR/Cas9 system is a defense mechanism used by bacteria to resist the invasion of viruses and foreign plasmids. At present, the type II CRISPR/Cas9 system is the most developed and widely used system. It recognizes the target sequence with gRNA, and guide Cas9 endonuclease to cut the upstream of PAM, resulting in the double-strand break (DSB) of the target site DNA. To repair the DSB, the cell uses its own DNA repair mechanism to add or delete or replace pieces of a DNA sequence via Homology Directed Repair (HDR) or Non-Homologous End Joining (NHEJ).
Ubigene developed a series of YKO plasmids, including lentivirus plasmids, AAV plasmids, and non-viral plasmids, which can be widely used in vitro or in vivo.
The plasmid can be used in frame-shift mutation or short fragment knockout. By co-transfecting 2-3 gRNAs, knockout efficiency can be improved.
Dual-gRNA plasmid can be used in large fragment removal. Dual-gRNA can greatly improve the efficiency of cell transfection and gene knockout.
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