Virus Packaging - AAV for KO, KI and over-expression in vitro and in vivo

Adeno-associated virus (AAV) is a kind of Parvovirus, which genome is single-stranded DNA. It can infect both divisive and non-divisive cells. Adenoviruses or herpes viruses are usually needed to assist AVV to replicate in vivo. Recombinant AAV (rAAV) is a viral vector that combines the AAV2 genome with capsid protein genomes of different serotypes. CDS or RNAi interference sequences of the target genes can be inserted into the rAAV plasmid. AAV is mainly used in vivo injection because it has low immunogenicity and long-term expression.

Ubigene's AAV will be purified by ultracentrifugation, and its genome copy will be titrated by qPCR. The titer of AAV ranged from 10^12 to 10^13 v. g. / ml.